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noti cloning sites  (TaKaRa)


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    Structured Review

    TaKaRa noti cloning sites
    Noti Cloning Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/noti+cloning+sites/Not+I/pmc12816902-221-18-24
    Average 97 stars, based on 4277 article reviews
    noti cloning sites - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Cloning:

    Article Title: Sorting of the Neuroendocrine Secretory Protein Secretogranin II into the Regulated Secretory Pathway
    Article Snippet: .. To design SgII domain-EAP fusion chimeras, EGFP was substituted by EAP at the KpnI and NotI cloning sites of each of the SgII domain/EGFP plasmids described above. pCMV-SgII-(254-304)-EAP and pCMV-SgII-(1181-1225)-EAP were obtained by inserting EAP into the BamHI and NotI cloning sites of EGFP. pECFP-Golgi (Clontech) encoding the membrane-anchoring region of glycoprotein β1,4-galactosyltransferase (GalT-CFP) was used to design pECFP-Golgi-SgII. .. The ochre codon of enhanced CFP (TAA at position 1581-1583) was mutated to a serine codon (TCA) by site-directed mutagenesis (QuikChange, Stratagene) to produce mutated pECFP-Golgi.

    Article Title: Targeted delivery of rhodopsin’s assembled core is required for outer segment extension in mouse rod photoreceptors
    Article Snippet: For rod-specific expression, complementary DNA sequences were cloned between 5′ AgeI and 3′ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed (gift from Dr Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156 ). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the cytomegalovirus promoter. .. The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma–Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma–Aldrich); goat polyclonal anti-FLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Article Title: Robo1 and Robo2 are homophilic binding molecules that promote axonal growth.
    Article Snippet: In the present study we show that following transfection in 3T3 cells, human Robo1 and Robo2 stimulate neurite outgrowth from Robo-positive neurons (retinal neurons and olfactory neurons), but have no effect on Robo-negative neurons (cerebellar granule cells).. The neurite outgrowth response was inhibited by an antibody raised against the first Ig domain of Robo1/2 or by soluble Robo-Fc chimera.. Furthermore, we show that the extracellular domains of Robo1 and Robo2 are homophilic adhesion molecules that can also interact with each other.

    Article Title: Targeted delivery of rhodopsin's assembled core is required for outer segment extension in mouse rod photoreceptors.
    Article Snippet: For rod-specific expression, cDNA sequences were cloned between 5’ AgeI and 3’ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed, (gift from Dr. Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the CMV promoter. .. Antibodies The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma-Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma-Aldrich); goat polyclonal antiFLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Article Title: Sorting of the Neuroendocrine Secretory Protein Secretogranin II into the Regulated Secretory Pathway
    Article Snippet: .. To design SgII domain-EAP fusion chimeras, EGFP was substituted by EAP at the KpnI and NotI cloning sites of each of the SgII domain/ EGFP plasmids described above. pCMV-SgII-(254–304)-EAP and pCMV-SgII-(1181–1225)-EAP were obtained by inserting EAP into the BamHI and NotI cloning sites of EGFP. pECFPGolgi (Clontech) encoding the membrane-anchoring region of glycoprotein 1,4-galactosyltransferase (GalT-CFP) was used to design pECFP-Golgi-SgII. .. The ochre codonof enhancedCFP (TAA at position 1581–1583) was mutated to a serine codon (TCA) by site-directed mutagenesis (QuikChange, Stratagene) to produce mutated pECFP-Golgi.

    Article Title: Targeted delivery of rhodopsin’s assembled core is required for outer segment extension in mouse rod photoreceptors
    Article Snippet: For rod-specific expression, cDNA sequences were cloned between 5’ AgeI and 3’ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed, (gift from Dr. Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156 ). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the CMV promoter. .. The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma-Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma-Aldrich); goat polyclonal anti-FLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Membrane:

    Article Title: Sorting of the Neuroendocrine Secretory Protein Secretogranin II into the Regulated Secretory Pathway
    Article Snippet: .. To design SgII domain-EAP fusion chimeras, EGFP was substituted by EAP at the KpnI and NotI cloning sites of each of the SgII domain/EGFP plasmids described above. pCMV-SgII-(254-304)-EAP and pCMV-SgII-(1181-1225)-EAP were obtained by inserting EAP into the BamHI and NotI cloning sites of EGFP. pECFP-Golgi (Clontech) encoding the membrane-anchoring region of glycoprotein β1,4-galactosyltransferase (GalT-CFP) was used to design pECFP-Golgi-SgII. .. The ochre codon of enhanced CFP (TAA at position 1581-1583) was mutated to a serine codon (TCA) by site-directed mutagenesis (QuikChange, Stratagene) to produce mutated pECFP-Golgi.

    Article Title: Sorting of the Neuroendocrine Secretory Protein Secretogranin II into the Regulated Secretory Pathway
    Article Snippet: .. To design SgII domain-EAP fusion chimeras, EGFP was substituted by EAP at the KpnI and NotI cloning sites of each of the SgII domain/ EGFP plasmids described above. pCMV-SgII-(254–304)-EAP and pCMV-SgII-(1181–1225)-EAP were obtained by inserting EAP into the BamHI and NotI cloning sites of EGFP. pECFPGolgi (Clontech) encoding the membrane-anchoring region of glycoprotein 1,4-galactosyltransferase (GalT-CFP) was used to design pECFP-Golgi-SgII. .. The ochre codonof enhancedCFP (TAA at position 1581–1583) was mutated to a serine codon (TCA) by site-directed mutagenesis (QuikChange, Stratagene) to produce mutated pECFP-Golgi.

    Expressing:

    Article Title: Targeted delivery of rhodopsin’s assembled core is required for outer segment extension in mouse rod photoreceptors
    Article Snippet: For rod-specific expression, complementary DNA sequences were cloned between 5′ AgeI and 3′ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed (gift from Dr Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156 ). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the cytomegalovirus promoter. .. The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma–Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma–Aldrich); goat polyclonal anti-FLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Article Title: Targeted delivery of rhodopsin's assembled core is required for outer segment extension in mouse rod photoreceptors.
    Article Snippet: For rod-specific expression, cDNA sequences were cloned between 5’ AgeI and 3’ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed, (gift from Dr. Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the CMV promoter. .. Antibodies The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma-Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma-Aldrich); goat polyclonal antiFLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Article Title: Targeted delivery of rhodopsin’s assembled core is required for outer segment extension in mouse rod photoreceptors
    Article Snippet: For rod-specific expression, cDNA sequences were cloned between 5’ AgeI and 3’ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed, (gift from Dr. Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156 ). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the CMV promoter. .. The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma-Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma-Aldrich); goat polyclonal anti-FLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Cell Culture:

    Article Title: Targeted delivery of rhodopsin’s assembled core is required for outer segment extension in mouse rod photoreceptors
    Article Snippet: For rod-specific expression, complementary DNA sequences were cloned between 5′ AgeI and 3′ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed (gift from Dr Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156 ). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the cytomegalovirus promoter. .. The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma–Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma–Aldrich); goat polyclonal anti-FLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Article Title: Targeted delivery of rhodopsin's assembled core is required for outer segment extension in mouse rod photoreceptors.
    Article Snippet: For rod-specific expression, cDNA sequences were cloned between 5’ AgeI and 3’ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed, (gift from Dr. Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the CMV promoter. .. Antibodies The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma-Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma-Aldrich); goat polyclonal antiFLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Article Title: Targeted delivery of rhodopsin’s assembled core is required for outer segment extension in mouse rod photoreceptors
    Article Snippet: For rod-specific expression, cDNA sequences were cloned between 5’ AgeI and 3’ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed, (gift from Dr. Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156 ). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the CMV promoter. .. The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma-Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma-Aldrich); goat polyclonal anti-FLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Molecular Cloning:

    Article Title: Targeted delivery of rhodopsin’s assembled core is required for outer segment extension in mouse rod photoreceptors
    Article Snippet: For rod-specific expression, complementary DNA sequences were cloned between 5′ AgeI and 3′ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed (gift from Dr Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156 ). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the cytomegalovirus promoter. .. The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma–Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma–Aldrich); goat polyclonal anti-FLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Article Title: Targeted delivery of rhodopsin's assembled core is required for outer segment extension in mouse rod photoreceptors.
    Article Snippet: For rod-specific expression, cDNA sequences were cloned between 5’ AgeI and 3’ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed, (gift from Dr. Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the CMV promoter. .. Antibodies The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma-Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma-Aldrich); goat polyclonal antiFLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Article Title: Targeted delivery of rhodopsin’s assembled core is required for outer segment extension in mouse rod photoreceptors
    Article Snippet: For rod-specific expression, cDNA sequences were cloned between 5’ AgeI and 3’ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed, (gift from Dr. Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156 ). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the CMV promoter. .. The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma-Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma-Aldrich); goat polyclonal anti-FLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Plasmid Preparation:

    Article Title: Targeted delivery of rhodopsin’s assembled core is required for outer segment extension in mouse rod photoreceptors
    Article Snippet: For rod-specific expression, complementary DNA sequences were cloned between 5′ AgeI and 3′ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed (gift from Dr Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156 ). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the cytomegalovirus promoter. .. The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma–Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma–Aldrich); goat polyclonal anti-FLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Article Title: Targeted delivery of rhodopsin's assembled core is required for outer segment extension in mouse rod photoreceptors.
    Article Snippet: For rod-specific expression, cDNA sequences were cloned between 5’ AgeI and 3’ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed, (gift from Dr. Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the CMV promoter. .. Antibodies The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma-Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma-Aldrich); goat polyclonal antiFLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Article Title: Targeted delivery of rhodopsin’s assembled core is required for outer segment extension in mouse rod photoreceptors
    Article Snippet: For rod-specific expression, cDNA sequences were cloned between 5’ AgeI and 3’ NotI cloning sites into a vector driven by a 2.2 kb bovine rhodopsin promoter originally cloned from pRho:DsRed, (gift from Dr. Connie Cepko; Addgene plasmid #11156; http://n2t.net/addgene:11156 ). .. For expression in cell culture, inserts from the pRho plasmids were transferred by molecular cloning with AgeI and NotI cloning sites into a pEGFP-N1 (Clontech) plasmid containing the CMV promoter. .. The following commercial primary antibodies were used: mouse monoclonal M2 anti-FLAG (F1804, Sigma-Aldrich); rabbit polyclonal anti-FLAG (F7425, Sigma-Aldrich); goat polyclonal anti-FLAG (ab1257, Abcam); rabbit polyclonal anti-FLAG (PA1-948B, Thermo Fisher); goat polyclonal anti-mCherry (AB0040, OriGene Technologies); rabbit polyclonal anti-Calnexin (C4731, Sigma); and mouse monoclonal anti-Na/K-ATPase (SC58628, Santa Cruz).

    Clone Assay:

    Article Title: Robo1 and Robo2 are homophilic binding molecules that promote axonal growth.
    Article Snippet: In the present study we show that following transfection in 3T3 cells, human Robo1 and Robo2 stimulate neurite outgrowth from Robo-positive neurons (retinal neurons and olfactory neurons), but have no effect on Robo-negative neurons (cerebellar granule cells).. The neurite outgrowth response was inhibited by an antibody raised against the first Ig domain of Robo1/2 or by soluble Robo-Fc chimera.. Furthermore, we show that the extracellular domains of Robo1 and Robo2 are homophilic adhesion molecules that can also interact with each other.

    Polymerase Chain Reaction:

    Article Title: Robo1 and Robo2 are homophilic binding molecules that promote axonal growth.
    Article Snippet: In the present study we show that following transfection in 3T3 cells, human Robo1 and Robo2 stimulate neurite outgrowth from Robo-positive neurons (retinal neurons and olfactory neurons), but have no effect on Robo-negative neurons (cerebellar granule cells).. The neurite outgrowth response was inhibited by an antibody raised against the first Ig domain of Robo1/2 or by soluble Robo-Fc chimera.. Furthermore, we show that the extracellular domains of Robo1 and Robo2 are homophilic adhesion molecules that can also interact with each other.

    Amplification:

    Article Title: Robo1 and Robo2 are homophilic binding molecules that promote axonal growth.
    Article Snippet: In the present study we show that following transfection in 3T3 cells, human Robo1 and Robo2 stimulate neurite outgrowth from Robo-positive neurons (retinal neurons and olfactory neurons), but have no effect on Robo-negative neurons (cerebellar granule cells).. The neurite outgrowth response was inhibited by an antibody raised against the first Ig domain of Robo1/2 or by soluble Robo-Fc chimera.. Furthermore, we show that the extracellular domains of Robo1 and Robo2 are homophilic adhesion molecules that can also interact with each other.

    cDNA Library Assay:

    Article Title: Robo1 and Robo2 are homophilic binding molecules that promote axonal growth.
    Article Snippet: In the present study we show that following transfection in 3T3 cells, human Robo1 and Robo2 stimulate neurite outgrowth from Robo-positive neurons (retinal neurons and olfactory neurons), but have no effect on Robo-negative neurons (cerebellar granule cells).. The neurite outgrowth response was inhibited by an antibody raised against the first Ig domain of Robo1/2 or by soluble Robo-Fc chimera.. Furthermore, we show that the extracellular domains of Robo1 and Robo2 are homophilic adhesion molecules that can also interact with each other.



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